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European heart journal
Published

Blood pressure, plasma proteins, and cardiovascular diseases: a network Mendelian randomization and observational study

Authors

Devendra Meena, Jingxian Huang, Alexander Smith, James Yarmolinsky, Siwei Wu, Fotios Koskeridis, Yi-Hsuan Ko, Marie-Joe Dib, Charalabos Antonatos, Yiannis Vasilopoulos, Xinzhu Yu, Georg W Otto, Dipender Gill, Manuel Mayr, Paul Elliott, Abbas Dehghan, Ioanna Tzoulaki

Abstract

Eur Heart J. 2025 Oct 9:ehaf725. doi: 10.1093/eurheartj/ehaf725. Online ahead of print.

ABSTRACT

BACKGROUND AND AIMS: The biological pathways leading to elevated blood pressure (BP) and subsequent cardiovascular diseases (CVDs) remain incompletely understood. Investigating the proteomic landscape of BP and its overlap with CVD could provide critical insights into the molecular determinants and pathways involved in BP regulation and its subsequent effect on CVD.

METHODS: A proteome-wide Mendelian randomization (MR) study was conducted by leveraging genetic instruments from 2007 plasma proteins to assess their causal effects on BP (systolic and diastolic BP). Proteins showing strong associations with BP were further analyzed for potential causal effects on coronary artery disease (CAD) and stroke subtypes. Network MR was performed to estimate the proportion of CVD risk mediated through BP. Bayesian colocalization was applied to determine whether identified associations share common causal variants. Observational associations were examined in UK Biobank participants to assess associations between proteins, BP, and incident CVD events using linear regression and Cox proportional hazard models.

RESULTS: Proteome-wide MR identified 242 proteins associated with BP, of which 48 were also linked to CAD or stroke, with four (ACOX1, FGF5, FURIN, MST1) also supported by genetic colocalization analyses (FDR 5% and PP ≥70%). Genetically predicted FURIN and FGF5 were strongly associated with BP and stroke risk, while ACOX1, FGF5, and MST1 exhibited potential causal effects on CAD. Network MR suggested that a substantial proportion of their effect on CAD and stroke (30.5%-77.2%) was mediated through BP regulation. Observational analyses further supported these findings.

CONCLUSIONS: This study identifies key plasma proteins with potential causal roles in BP regulation and CVD risk, highlighting BP as a major mediator of their effects on CAD and stroke. These findings provide novel insights into the molecular mechanisms underlying hypertension-related CVD and identify promising protein targets for further investigation.

PMID:41065563 | DOI:10.1093/eurheartj/ehaf725

UK DRI Authors

Paul Elliott

Prof Paul Elliott

Group Leader

Using advanced methods in genetic, epidemiology and metabolic phenotyping to improve understanding of dementias

Prof Paul Elliott