Abstract
Proc Natl Acad Sci U S A. 2025 Oct 14;122(41):e2422878122. doi: 10.1073/pnas.2422878122. Epub 2025 Oct 7.
ABSTRACT
Activating the adra2a receptors of the brain's norepinephrine (NE) system produces NREM-like sleep and hypothermia. Dexmedetomidine (DEX), a selective α2 receptor (adra2a) agonist, is an important drug used in intensive care for arousable sedation. But for some patients, even slight decreases in body temperature during sedation could pose health risks such as increasing the likelihood of postoperative cognitive dysfunction. Where are the adra2a receptors at which DEX acts to induce hypothermia? A popular theory is that inhibitory adra2a receptors expressed on norepinephrinergic locus coeruleus (LC) neurons are DEX's prime targets. To examine this, we genetically lesioned galanin (gal)-expressing norepinephrinergic LC neurons in adult mice. In these ΔLC mice, baseline sleep-wake levels were unchanged, but surprisingly, core body temperatures were lower. Maintaining body temperature depended on NE/gal projections from the LC to the medial preoptic (MPO) hypothalamus. Furthermore, without these LC neurons, DEX induced hypothermia more strongly. Knockdown with adra2a shRNA revealed that DEX induces hypothermia via adra2a receptors on MPO glutamate cells. When ΔLC mice were kept warm, DEX could induce the same amount of NREM-like sleep as well as it did for mice with an intact LC, and this sleep-like state in ΔLC mice had a higher delta frequency power than in control mice. Our results emphasize that inhibition of the LC by DEX is not required to induce robust sedation. By contrast, the LC likely has a fortuitous background action in protecting against drug-induced hypothermia and speeds recovery from hypothermia.
PMID:41055995 | DOI:10.1073/pnas.2422878122
UK DRI Authors
