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Resuscitation
Published

Neurofilament light chain evaluated with a new automated commercially available method for outcome prediction in out-of-hospital cardiac arrest patients

Authors

Christel Pussinen, Mikko Anttonen, Lauri Wihersaari, Marjaana Tiainen, Matti Reinikainen, Johanna Hästbacka, Henrik Zetterberg, Kaj Blennow, Kari Pulkki, Markus B Skrifvars

Abstract

Resuscitation. 2025 Jun 27:110698. doi: 10.1016/j.resuscitation.2025.110698. Online ahead of print.

ABSTRACT

AIM: of the study: The biomarker neurofilament light (NfL) measured from plasma predicts outcome at 12-72 h after out-of-hospital cardiac arrest (OHCA). We performed a post-hoc analysis of NfL using samples from 112 patients in the COMACARE trial, applying the Siemens Atellica NfL.

METHODS: We analyzed samples collected between 2016 and 2017 using the Siemens Atellica NfL and compared the results with those from the Quanterix Simoa NF-Light-method. We defined poor outcome as Cerebral Performance Category (CPC) 3-5 at six months after OHCA. We evaluated the accuracy of NfL measured at 24-72 h for outcome prediction with high specificity. We calculated the areas under the receiver operating characteristic curves (AUROC) and compared the results of the two methods.

RESULTS: The Atellica NfL showed high prognostic performance, with AUROCs (0.97-0.98) comparable to those of the Simoa NfL method with a minimal difference (-0.008-0.001). Tentative cut-off values based on this limited sample size for predicting poor outcome at six months after OHCA with 99% specificity were 112, 229, and 331 ng/L at 24, 48 and 72 h, respectively. Cut off values for predicting good outcome with 100% sensitivity were below 29 ng/L at 24, 48, and 72 h .

CONCLUSION: The Atellica NfL assay accuracy appears well comparable to that obtained using the more laborious Simoa method. Further larger studies are needed to determine cut-off values in heterogenous populations of cardiac arrest patients.

PMID:40582485 | DOI:10.1016/j.resuscitation.2025.110698

UK DRI Authors

Profile picture of Henrik Zetterberg

Prof Henrik Zetterberg

Group Leader

Pioneering the development of fluid biomarkers for dementia

Prof Henrik Zetterberg