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Scandinavian journal of clinical and laboratory investigation
Published

Stability of bilirubin and oxyhaemoglobin in cerebrospinal fluid

Authors

Marcus Clarin, Alexandra Hellberg, Kaj Blennow, Ulf Andreasson, Henrik Zetterberg

Abstract

Scand J Clin Lab Invest. 2024 Dec 23:1-5. doi: 10.1080/00365513.2024.2442511. Online ahead of print.

ABSTRACT

Cerebrospinal fluid (CSF) is routinely investigated to diagnose subarachnoid haemorrhage (SAH) in cases with unclear neuroimaging findings. Using spectrophotometry, the levels of bilirubin and oxyhaemoglobin are analysed. This study investigates the stability for bilirubin and oxyhaemoglobin in CSF samples for up to 3 weeks measured with a spectrophotometer. The absorbances corresponding to bilirubin (455 nm) and oxyhaemoglobin (415 nm) remained fairly stable for up to 3 weeks when samples were stored at +4 °C with light protection. There was a statistically significant trend of decreased absorbance for both oxyhaemoglobin and bilirubin already after exposure to light within 120 min from sampling. It is therefore advisable to protect CSF from light until spectrophotometric analysis.

PMID:39714981 | DOI:10.1080/00365513.2024.2442511

UK DRI Authors

Profile picture of Henrik Zetterberg

Prof Henrik Zetterberg

Group Leader

Pioneering the development of fluid biomarkers for dementia

Prof Henrik Zetterberg