We are seeking a Research Technician to contribute to a project involving the analysis of human post-mortem brain tissue and various cell culture models, including iPSC-derived and primary human neurons, as well as structural in vitro assays using recombinant protein. Both native brain tissue and in vitro models will be used to study α-synuclein fibrils across different synucleinopathies.
In vitro aggregation assays, electron microscopy, circular dichroism (CD) spectroscopy, and fluorescence correlation spectroscopy (FCS) will be employed to elucidate context-dependent protein folding. You will provide support with histopathology techniques, biochemical analyses such as crosslinking and Western blotting, cell culture, and protein expression and purification.
You will have an Honours degree (minimum 2:1) or equivalent in Biophysics, Biochemistry, Molecular Biology or a related discipline, and experience with iPSC culture and neuronal differentiation.
A background and knowledge in one or more of the following is essential: Neurodegeneration, synucleinopathies, biochemical analysis, such as detergent extraction and Western Blot, cell culture experience, IHC.
Experience of working with Biophysics, Biochemistry, Molecular Biology, Histology assays is also a requirement, as is an understanding of the mission of the UK DRI, an interest in its research work and a commitment to supporting high quality research.
Key details
- Location UK DRI at UCL
- Salary: £35,930-£41,255
- Lab: Tim Bartels